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Health for purpose in constructed wetlands
Expanded Title:Constructed wetlands (CWs) form low-maintenance, cost effective wastewater treatment systems that are environmentally friendly and aesthetically pleasing. The main objective of the research was to evaluate the potential of maintaining a viable “healthy” microbial population capable of degrading target pollutants within pilot-scale constructed wetlands treating specifically winery wastewater. Within the scope of this project, two microbial fingerprinting methods (DGGE and T-RFLP) were modified and developed to study population changes in the systems. Using T-RFLP, DGGE and standardized influent and effluent analyses, it was demonstrated that autochthonous microbial communities within the CW were modified and were able to adapt to amendment with ethanol, winery wastewater and synthetic phenolic wastewater. The study demonstrated both temporal and spatial adaptation of these communities. Furthermore, a second procedure called incremental priming where increasing concentrations of ethanol was used for amendment of the system also resulted in an increase in biodegradative efficiency. From these conclusions simple guidelines were recommended for implementation in full-scale systems and/or in a research environment in order to maintain optimal microbial “health”.
Date Published:01/02/2012
Document Type:Research Report
Document Subjects:Wastewater Management - Industrial
Document Keywords:Municipality
Document Format:Report
Document File Type:pdf
Research Report Type:Standard
WRC Report No:1936/1/11
ISBN No:978-1-4312-0224-9
Authors:Burton SG; Welz PJ; Ramond J-B; Sheridan C; Kirby B; Schueller A; Rodriguez A; Pather-Elias S; Prins A; Cowan DA
Project No:K5/1936
Originator:WRC
Organizations:Biocatalysis and Technical Biology Research Group, Cape Peninsula University of Technology; Burton Research Group, University of Cape Town; The Institute for Microbial Biotechnology and Metagenomics,University of the Western Cape
Document Size:9 458 KB
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