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Enrichment and characterization of indigenous anaerobic ammonium oxidizing (anammox) bacteria from wastewater treatment plants in Tshwane, South Africa
Expanded Title:In this study, a process for the start-up and enrichment of anammox bacteria from wastewater treatment was developed and tested in laboratory batch reactors. Anammox biomass was successfully enriched from the sludge samples from sludge samples with very low source anammox concentration. The anammox cultures were enriched from three sludge sources from treatment plants around Pretoria. Identification of anammox bacteria in batch reactors was done by PCR analysis. These results showed that anammox bacteria are ubiquitous in wastewater treatment plants in South Africa. The detected anammox bacteria belonged to the Brocadia species (fulgida and anammoxidans). Sequencing batch reactor (SBR) was set up and operated for the removal of nitrogen compounds in wastewater. Samples of biomass from successfully enriched cultures were used as inoculum for the SBR. The systems worked effectively, achieving the nitrite removal rate of 97 %. Samples of biomass obtained from the SBR were analysed for identification of the main bacterial populations present in the reactor. Next generation sequencing (NGS) was used for identification of bacterial population. The reactor was found to be dominated by different bacillus species with abundance of 84%. Although the overabundance of these bacterial species is not clear, it could be associated with the nature of the bacteria of being very resistant and able to survive in most environmental conditions. Additionally, their involvement in the nitrogen assimilation process could also explain their over-abundance in the reactor. However, the total of 5% anammox and planctomycetes bacteria was also seen in the reactor. Nitrosomonas species which are known ammonia oxidizers were also present in the reactor though in very small amounts (0.2%).
Date Published:01/12/2014
Document Type:Research Report
Document Format:Report
Document File Type:pdf
Research Report Type:Standard
WRC Report No:2117/1/14
ISBN No:978‐1‐4312‐0613‐1
Authors:Tikilili PV; Chirwa EMN
Project No:K5/2117
Originator:WRC
Organizations:University of Pretoria
Document Size:827 KB
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