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Rapid enzymatic detection of organochlorine pesticides in water
Expanded Title:Interest in environmental issues has highlighted the problem of pesticides in the environment, prompting a need for effective methods of detection. Pesticides detected in water and sediments include pyrethroids, organochlorines (DDT, DDE), organophosphates and carbamates. Current methods can offer detection; however, they have limitations. Enzymatic methods offer a simpler, more rapid and cost effective alternative. Enzyme inhibition by pesticides can be monitored using assays, the degree of inhibition being proportional to the concentration of pesticide. Such enzymes include alkaline phosphatases (ALP). However, only a limited number of contaminants have been tested using enzymatic methods and there is therefore a need to determine the effect of a range of contaminants on enzyme activity. This study showed that ALP was not an effective target for a biosensor for pesticides, however Cd, Hg, Ni and Zn were investigated and had a dramatic effect on the ALP, increasing its relative activity by 200% even at low concentrations. ALP activity was enhanced in the presence of environmental samples (Eastern Cape rivers), indicating the potential presence of metal ions. Therefore ALP is more suitable as an target for the detection and monitoring of metals in water.
Date Published:01/10/2010
Document Type:Research Report
Document Subjects:Agricultural Water - Commercial irrigation, Wastewater Management - Agricultural
Document Keywords:Environment, Pollution control, Surface Water
Document Format:Report
Document File Type:pdf
Research Report Type:Consultant
WRC Report No:KV 252/10
ISBN No:978-1-4312-0040-5
Authors:Pletschke B; Cockburn I; Adebiyi P; Van Dyk JS
Project No:K8/828
Organizations:Rhodes University
Document Size:368 KB
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